FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000931861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTA80570.40285TruSeq Adapter, Index 1 (97% over 36bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTA49490.24745Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA43070.21535No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39310.19654999999999997No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTAT23340.11670000000000001TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)