FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG111320.5566No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA104340.5217No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA89210.44605No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT69500.3475No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA67450.33725No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG44610.22304999999999997No Hit
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC43580.21789999999999998No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT40370.20185No Hit
GGGTGGATCACAAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGA33770.16885No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC32870.16435No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA32850.16425No Hit
CTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGGTTG32750.16375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTAT31920.1596TruSeq Adapter, Index 5 (97% over 38bp)
CCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACAC27670.13835No Hit
CTCCAGCATTGTAGTTTGTAGCTCGTGTGTTGTAACCACTCTCCCATTTG27470.13735No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGATCTGATCTCGTAT26830.13415Illumina Single End PCR Primer 1 (96% over 25bp)
GGCGTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTG22910.11455No Hit
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACC21880.1094No Hit
GCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAG20960.1048No Hit
CCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAG20690.10345No Hit
GGGCATAATCGTATCATTCAAGATTAAGTCTGGGTTATTTCTTTGATAAG20190.10095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)