FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000931945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA104180.5209No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT78270.39135000000000003TruSeq Adapter, Index 25 (97% over 37bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC65800.329No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT53830.26915No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT39250.19624999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC37660.1883No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT34700.1735No Hit
GCTCCGTCCTACTCGATTTCATGACTAAGAGATTTTCGCGTACAGGGCTA29720.14859999999999998No Hit
CCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCTC27340.1367No Hit
CCCCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTCT24180.12090000000000001No Hit
GCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGAC21880.1094No Hit
CCCCCATTCAGACATCTCCGGATCAAAGTCTGTTTGCCGACTCCCCGAAG21520.10759999999999999No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTGGTCAATCTCGTAT21380.10690000000000001Illumina Single End PCR Primer 1 (96% over 25bp)
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG20950.10475000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)