FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000931963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000931963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA272721.3636000000000001No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC163110.81555No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC83480.4174No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT79760.39880000000000004TruSeq Adapter, Index 1 (97% over 38bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA72710.36355No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC62050.31024999999999997No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT58590.29295No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG53690.26844999999999997No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC49120.24559999999999998No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA48550.24275No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT44060.2203No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG41580.2079No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT39220.1961No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT37000.185Illumina Single End PCR Primer 1 (96% over 25bp)
GTGGGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGA36740.1837No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG36340.1817No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT32330.16165000000000002No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA32040.16019999999999998No Hit
GCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGA32010.16005No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA31160.1558No Hit
GTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCGTCC30100.1505No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT29830.14915No Hit
GGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATC29430.14715No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT29130.14565No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG28860.1443No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC28220.1411No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT27810.13905No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC27200.136No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG27190.13595000000000002No Hit
GCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAA26840.1342No Hit
GGGGGTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTT26370.13185No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC25890.12945No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT25680.1284No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG25420.1271No Hit
GTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAG25140.1257No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC24790.12394999999999999No Hit
GTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAA24070.12035No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT23680.11839999999999999No Hit
GGGGGCTTCGACATGGGCTTTAGGGAGTCATAAGTGGAGTCCGTAAAGAG23560.1178No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT23470.11735000000000001No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA23330.11665No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG22350.11175No Hit
GCCGATGTTTCAGGTTTCTGAGTAGAGAAATGATCCGTAATATAGGCCTC21960.10980000000000001No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT20560.1028No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG20280.10139999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)