FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000932176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000932176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTA71320.35660000000000003Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTA50750.25375TruSeq Adapter, Index 1 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA44210.22104999999999997No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39380.1969No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTAT22690.11345000000000001TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)