FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000932352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000932352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA279651.39825No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC169920.8496No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC83560.4178No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT80630.40315TruSeq Adapter, Index 1 (97% over 38bp)
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA75540.3777No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC61570.30785No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT59440.29719999999999996No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA51610.25805No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC51320.2566No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG49950.24975000000000003No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT44010.22005000000000002No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG43740.2187No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT40760.20379999999999998No Hit
GTGGGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGA38840.19419999999999998No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG38650.19325No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA34710.17355No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA33800.169No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT33700.1685No Hit
GCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGA33550.16774999999999998No Hit
GTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCGTCC31860.1593No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT31060.1553No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT31050.15525No Hit
GGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATC30340.1517No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT29430.14715Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT28570.14285No Hit
GGGGGTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTT28180.1409No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC28110.14055No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC28040.1402No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG27760.13879999999999998No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG27450.13725No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC27320.1366No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC26030.13015No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT25640.12819999999999998No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG25290.12645No Hit
GCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCTGTTTTTAA25280.12639999999999998No Hit
GTTGTGGTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAG25210.12605No Hit
GTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAA25080.12539999999999998No Hit
GGGGGCTTCGACATGGGCTTTAGGGAGTCATAAGTGGAGTCCGTAAAGAG24590.12294999999999999No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT24370.12185No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT23590.11795No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA23210.11605000000000001No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG22990.11495No Hit
GCCGATGTTTCAGGTTTCTGAGTAGAGAAATGATCCGTAATATAGGCCTC22290.11145000000000001No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG21020.10510000000000001No Hit
GTCAGGGGGTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAG20560.1028No Hit
GGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTAT20250.10124999999999999No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG20160.1008No Hit
GTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTGCGGCGGGTAG20020.1001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)