FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000932356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000932356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA220101.1004999999999998No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC111730.55865No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT59330.29664999999999997TruSeq Adapter, Index 1 (97% over 38bp)
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC48530.24264999999999998No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG43660.2183No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA43560.2178No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT42840.21419999999999997No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC42070.21034999999999998No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC37560.1878No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT31120.15560000000000002Illumina Single End PCR Primer 1 (96% over 25bp)
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT29650.14825000000000002No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA29340.1467No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG28200.14100000000000001No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT24650.12325000000000001No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT24130.12065000000000001No Hit
GCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGA23830.11915No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG23810.11904999999999999No Hit
GTGGGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGA23330.11665No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG21880.1094No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC21550.10774999999999998No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT21320.10660000000000001No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA20780.10389999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)