FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000932414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000932414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT178910.8945500000000001No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT83740.41869999999999996No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA77220.3861No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT72720.3636No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA68890.34445000000000003No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT59780.2989No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG57720.2886No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC56770.28385No Hit
GGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT52800.264No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC51610.25805No Hit
CGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT50320.2516No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA49860.2493No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT48620.2431No Hit
CAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTC40140.20070000000000002No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA39170.19585000000000002No Hit
GCAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCT35440.1772No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA31210.15605No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA30960.1548No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT29690.14845No Hit
GCTGCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTG28960.1448No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT27820.1391No Hit
CGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTTAC26110.13055No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC25130.12565No Hit
GGGGTCTTTTGCTCTTAAAATGAAGTGAAGCTTTGCAAGTCCATTGTCCA24760.1238No Hit
GCTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGA24520.12260000000000001No Hit
GTGGGATTTTTCCAACAGAGGTTTTTCACAGCATTCCTTCAGTTTACTGG24190.12095No Hit
CTGGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCG24190.12095No Hit
CTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCAAG24100.1205No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC23500.11750000000000001No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC22410.11205No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT22260.11130000000000001TruSeq Adapter, Index 20 (97% over 38bp)
CTTCTGCACAGGGCATTCTTTTTGCTTCAGGATGTTTACAACATTTGCTG22010.11005No Hit
GTTCCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTAT21540.1077No Hit
GCCGTGGTCAGCAGCCATTGTTCATTGATCAGCGTGGCACCTGTGGTGAG21310.10655No Hit
AGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTCA21170.10585No Hit
GGCACACTTGAGTCTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAA20960.1048No Hit
GCACATCACATCAACCTCTGGTCTCACCAATCGGGGGAGGTTTGGGTTGT20920.1046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)