FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000932416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000932416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT121250.6062500000000001No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT74360.3718No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA65630.32815No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT63760.3188No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA61440.30720000000000003No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT60290.30145No Hit
CTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGAG52480.2624No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC51800.259No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT50300.2515No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT45680.2284No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44190.22095No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA40730.20365No Hit
GGTGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTTT33250.16624999999999998No Hit
GCAGCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCT32520.1626No Hit
CGTGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTTT32220.1611No Hit
GGCGTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATA31130.15564999999999998No Hit
CTCTGTTTGGCAGACGAAGCCTTCCCTTCATCCCGAAGTTCATCGAGCTT28870.14434999999999998No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA28620.1431No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA28510.14254999999999998No Hit
CATCGGCACAGCACTTCTCTAGAGTGGTTTCATATGTCTTGGCAAGTCTC26570.13285No Hit
GCTGCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTG25860.1293No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT24720.12359999999999999No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC22400.11199999999999999No Hit
CCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACAC21630.10815No Hit
GCTTCAGGATGTTTACAACATTTGCTGCCCACTTTTCCTAGGTTTCTTGA21140.1057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT20860.1043TruSeq Adapter, Index 20 (97% over 38bp)
CGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTTAC20780.10389999999999999No Hit
CTTCTGCACAGGGCATTCTTTTTGCTTCAGGATGTTTACAACATTTGCTG20700.1035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)