FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT125990.62995TruSeq Adapter, Index 1 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA68930.34465No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC60050.30025No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTA44680.2234TruSeq Adapter, Index 1 (97% over 38bp)
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT41300.2065No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC29020.1451No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA26710.13355No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC25120.1256No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC22990.11495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)