FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000934183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465045
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTAT23680.16163326041179624TruSeq Adapter, Index 2 (97% over 38bp)
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT22340.15248678368241247No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTA21010.143408564242054TruSeq Adapter, Index 2 (97% over 38bp)
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA14670.10013344300004437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)