FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000934350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218254
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT9350.4283999376872818TruSeq Adapter, Index 1 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA7300.33447267862215585No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT3770.17273452032952433No Hit
GCCTGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC3590.16448724880185472No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA3080.14111997947345753No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC2850.13058179918810192No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC2530.11591998313891154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)