FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000934735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196906
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT20750.17336365595961586Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTAT15220.12716119728700498TruSeq Adapter, Index 14 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA14490.12106213854722091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)