FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA101240.5062No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC74840.37420000000000003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT47650.23825000000000002TruSeq Adapter, Index 1 (97% over 36bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC42380.21189999999999998No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT34560.17279999999999998Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC29460.1473No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT25400.127No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC25320.1266No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG23500.11750000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)