FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCC177490.8874500000000001No Hit
GCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCA118980.5949No Hit
GGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGG116820.5841No Hit
CCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCA110140.5507No Hit
TCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCC83120.41559999999999997No Hit
GGCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTC76170.38084999999999997No Hit
CACTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAG64100.3205No Hit
GGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGA57960.2898No Hit
CGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGG56530.28265No Hit
GGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCT53540.2677No Hit
CCACCTCAGAAATGCTGTGCTTCTCGACAGGCTCGCACAGTGGCCGGGGC43880.2194No Hit
GCGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGG43850.21925No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC43330.21665No Hit
GCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAACA36420.18209999999999998No Hit
GGCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAAC33090.16544999999999999No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC32690.16345No Hit
GGTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCA31580.1579No Hit
CGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCATC31180.1559No Hit
CCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCAA31040.1552No Hit
GTCCGGTTTAATTTTTGACTCCGTTGTAATTGCTGGATCAGTTCTAGACA27050.13525No Hit
CCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCAT25860.1293No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA24710.12355000000000001No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT24190.12095TruSeq Adapter, Index 15 (97% over 36bp)
GGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGAA23580.11789999999999999No Hit
GGACAGCGGATCCGTGCAGAGGGCAGTTCCCTCTGGGGTTCTTCACCTTG21760.1088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)