FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCC167520.8376No Hit
CCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCA105960.5297999999999999No Hit
GCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCA97260.4863No Hit
GGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGG95730.47865No Hit
TCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCC78980.39490000000000003No Hit
GGCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTC74070.37035No Hit
CACTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAG62470.31235No Hit
GGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGA52890.26445No Hit
CGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGG46480.23240000000000002No Hit
GGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCT42720.21359999999999998No Hit
CCACCTCAGAAATGCTGTGCTTCTCGACAGGCTCGCACAGTGGCCGGGGC42080.2104No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC40590.20295000000000002No Hit
GCGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGG36160.1808No Hit
GGCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAAC30970.15485No Hit
GCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAACA30510.15255No Hit
GGTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCA30290.15145No Hit
CCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCAA29570.14785No Hit
CGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCATC26990.13495000000000001No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC26660.1333No Hit
GTCCGGTTTAATTTTTGACTCCGTTGTAATTGCTGGATCAGTTCTAGACA23920.1196No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT23540.11770000000000001TruSeq Adapter, Index 15 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA22550.11275No Hit
GGACAGCGGATCCGTGCAGAGGGCAGTTCCCTCTGGGGTTCTTCACCTTG21190.10594999999999999No Hit
CCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCAT21000.105No Hit
GGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGAA20170.10085000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)