FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCC158100.7905000000000001No Hit
GCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCA106840.5342No Hit
CCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCA100870.5043500000000001No Hit
GGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGG88850.44425000000000003No Hit
TCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCC78620.39309999999999995No Hit
GGCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTC69880.3494No Hit
CACTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAG58380.2919No Hit
GGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGA45960.2298No Hit
CGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGG42540.2127No Hit
GGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCT40610.20305No Hit
CCACCTCAGAAATGCTGTGCTTCTCGACAGGCTCGCACAGTGGCCGGGGC38700.1935No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC38450.19225No Hit
GCGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGG33740.1687No Hit
GCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAACA33500.1675No Hit
GGCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAAC31130.15564999999999998No Hit
CCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCAA29390.14695No Hit
GGTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCA28210.14105No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC25560.1278No Hit
GTCCGGTTTAATTTTTGACTCCGTTGTAATTGCTGGATCAGTTCTAGACA24350.12175000000000001No Hit
CGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCATC22570.11284999999999999No Hit
GGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGAA22490.11245000000000001No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA22070.11035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT21580.10790000000000001TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)