FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCC170740.8536999999999999No Hit
GCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCA108410.54205No Hit
CCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCA107820.5391No Hit
GGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGG103800.519No Hit
TCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCC79980.3999No Hit
GGCCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTC74600.373No Hit
CACTCCCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAG62070.31035No Hit
GGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGA55440.2772No Hit
CGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGG54010.27005No Hit
GGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCT48610.24305No Hit
CCACCTCAGAAATGCTGTGCTTCTCGACAGGCTCGCACAGTGGCCGGGGC42710.21355No Hit
GTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCAC41940.2097No Hit
GCGGGGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGG40660.2033No Hit
GCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAACA31990.15995No Hit
GGCCGTATGACAAAATGAAGAGACTGAAATGACAGCGGGGAGGAAGAAAC31960.1598No Hit
GGTCAGGTTGGGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCA30670.15335No Hit
CCAACCTTCTCCATTGGGCTCGGTGCCCTGTGTGGGGTCATGGAGCCCAA30460.1523No Hit
CGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCATC28930.14465No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACACTGGTCTC28750.14375No Hit
GTCCGGTTTAATTTTTGACTCCGTTGTAATTGCTGGATCAGTTCTAGACA25690.12845No Hit
CCGGGGCTTGAGATGGTGGAGGGATTTCTCTCCCATGCAAAGTAATTCAT24020.1201No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA23360.1168No Hit
GGAGGAAGAAACAGAAGAAAGATAAGAATGAGGTGGTCAGGTTGGGGGAA22900.11449999999999999No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT22240.1112TruSeq Adapter, Index 15 (97% over 36bp)
GGACAGCGGATCCGTGCAGAGGGCAGTTCCCTCTGGGGTTCTTCACCTTG21200.106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)