FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000934823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT71170.35585Illumina Single End PCR Primer 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA70830.35415TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT67730.33865TruSeq Adapter, Index 5 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA59750.29875No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC51010.25505No Hit
CCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTT30810.15405No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA23430.11715No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT21320.10660000000000001No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC20530.10265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)