FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000934827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT75820.3791Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT38080.1904TruSeq Adapter, Index 5 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA37960.1898No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC33940.1697No Hit
CCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTT26940.13470000000000001No Hit
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA24060.1203Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)