FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000934829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT66500.33249999999999996Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT62790.31395TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA55540.2777TruSeq Adapter, Index 5 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA55510.27755No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC45570.22785000000000002No Hit
CCCAAATTCTAAGCAGAGTATGTAAATTGGAAGTTAACTTATGCACGCTT30430.15215No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA20820.1041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)