FastQCFastQC Report
Thu 9 May 2024
EGAF00000934867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000934867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases200 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA40130.20065No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC37430.18714999999999998No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTACAAGATCTCGTAT35800.179Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTAT32170.16085TruSeq Adapter, Index 14 (97% over 38bp)
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA24940.1247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)