FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000935016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176365
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTA6580.3730898987894424TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTAT4000.2268023700847674TruSeq Adapter, Index 2 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTA3480.19731806197374763TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)