FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000935050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA78060.3903No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC55900.2795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT36090.18045TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT32970.16485Illumina Single End PCR Primer 1 (96% over 25bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC26200.131No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC23690.11845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)