FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000935087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG99440.4972No Hit
CAAGCAGAAGACGGCATACGAGATCAGATCGGAAGAGCGTCGTGTAGGGA62620.3131Illumina PCR Primer Index 9 (96% over 31bp)
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG40700.2035No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA37760.1888No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG33180.16590000000000002No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG29190.14595No Hit
AGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTA28590.14295No Hit
ACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACC27670.13835No Hit
GAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGG26560.1328No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAGA24740.1237No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT24720.12359999999999999No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT23690.11845No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAG22050.11025No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG21810.10905000000000001Illumina Single End PCR Primer 1 (100% over 30bp)
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA21420.10709999999999999No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG20990.10495000000000002No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG20530.10265No Hit
CGGAGGAGTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTCGTAGAGT20410.10204999999999999No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA20170.10085000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)