FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000935099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG112240.5612No Hit
CAAGCAGAAGACGGCATACGAGATCAGATCGGAAGAGCGTCGTGTAGGGA106500.5325Illumina PCR Primer Index 9 (96% over 31bp)
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG44240.2212No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA41200.20600000000000002No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG39590.19795Illumina Single End PCR Primer 1 (100% over 30bp)
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG33930.16965000000000002No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG32170.16085No Hit
AGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTA30840.1542No Hit
ACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACC29630.14815No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT28930.14465No Hit
GAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGG27840.1392No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAGA26760.1338No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT25030.12515No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGAGAG23510.11754999999999999No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC22930.11465Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG22650.11325No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA22170.11084999999999999No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA21850.10925No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG21820.1091No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT21380.10690000000000001Illumina Single End PCR Primer 1 (100% over 31bp)
CGGAGGAGTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTCGTAGAGT20550.10275No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC20420.1021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)