FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000935329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT498482.4924TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA157150.78575TruSeq Adapter, Index 5 (97% over 36bp)
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT55370.27685000000000004No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA47310.23655No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA40280.20140000000000002No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT38550.19275No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC37830.18914999999999998No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC32910.16455No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA31300.1565No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC27610.13805No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT26320.1316No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT24580.12290000000000001No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA22870.11435No Hit
GGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAAT22710.11355No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA22280.1114No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT20510.10254999999999999No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT20410.10204999999999999No Hit
GCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGA20040.10020000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)