FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000935419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG89060.44530000000000003No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA71840.3592No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC41140.2057No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA40250.20124999999999998No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA38360.1918No Hit
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA34270.17135No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT31720.1586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT27830.13915TruSeq Adapter, Index 25 (97% over 38bp)
GCCTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAAC25990.12995No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG22530.11264999999999999No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT22450.11225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)