FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000935654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508
Sequences flagged as poor quality0
Sequence length296-300
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCTGCGTGGCGATCAGGCCGGCCCCCGGGCTGGTGCTGAGAGGCCGC1807.177033492822966No Hit
CTCGGCAGGCAGCAGCACGTGGAGCCCACGGCGCGGCAGCGGCACTGGCT1767.017543859649122No Hit
GTGCTGGCGGCAAGACGAGCTCTCGGAGGATGCTGCGGCGCGGGGCGCGT1465.821371610845295No Hit
GGGCCGCCGTCACAAACTCTGTAGCCCTGACCCGGGCCAGGCCAACAACA1305.18341307814992No Hit
TCCCTGGGCTTCCACAGCAGCCCCTGCCTGCCTGGCGGGACCCCACGTCC1074.2663476874003186No Hit
CGCCGAGGCGAATCCTCACATTGCGGCGTGGTGGCCGACAAAACCCGGGA993.9473684210526314No Hit
GGAGCCACGGGCACCTACCAGCATCCCTGGGGGTGGGCAGGGCTTGGTGC943.7480063795853265No Hit
AAGCGGAGGGCAGCAAAGCCCCCGAATGATTTCGCTTACCCCCACCCGCC843.349282296650718No Hit
AAGCCGCAGCAGAGATGGGAGTACAGGGAGGGGAGGTCCCAATGCCCGCT833.3094098883572567No Hit
CTAGCGCCTTCTGCCTCCTCTTCTTGGCTCTCCTCCTCGCTGTCCTCCTG823.269537480063796No Hit
GCCTGAGGGACACCCAGAGCTTCGGCGGAGCGGAGCGCGGTGCACAGAGC823.269537480063796No Hit
GCGCAGCGAGGGATCAGAAGGTCTCGGACGCCGCGGCAGGGCCGGCCAGG773.070175438596491No Hit
GGGCCTTCCCTTCACAGGCCCCGCAGACACCAGCCCAGGACCCGGAGGCC763.0303030303030303No Hit
GCCTTCGCCGCACACAAGCTCGTGTCTGTGGGTCCGTGTCGGGGGCTCAC732.9106858054226477No Hit
AGACCCACGCATACGAGTCCAGGGGCTGCTACAAAGCGCGGCGACGTCTC702.7910685805422646No Hit
AAGCGGGACGAGGAGAAGAAAAACCACTACTCGTACGGCTGGTCCTTCTA602.3923444976076556No Hit
GCTGGGTCCACCTCTCCCTCTGCCACTCCCTTGTAGAAGGAGCCTGTGGG552.1929824561403506No Hit
CCCAGCCAGCCTGCATTCCAGGTCTCAGATCCCTGCAGACCACCCTGGGG542.15311004784689No Hit
TGATCAGCACCGCGGACAGCGGCGGCGCAGGCAGCGGGCCGGGGGCGGGG501.9936204146730463No Hit
GGGTCACCAGGGCTCCAGCTCTGGGAGGGGCAGGATGGGGGCCCGTGGTG481.9138755980861244No Hit
TCTGCGGAGGGCTGCTTCTACAACGCTGACTACCTGGCGGCCCGAGCCCG441.7543859649122806No Hit
CGCTACCCTCCTCACACACCTCGGTCCCATAACTACCCATTTCCCGGTCA441.7543859649122806No Hit
CTGAGCTTCCCAGCGAGTCCCATGCAACCCTCAGCCGGGCGGCCCCCGGA431.7145135566188199No Hit
GGCAGAAGGGCCAGAACGGCTCCTCAGCCTTACCTCCCACTAGTGTGGGG431.7145135566188199No Hit
GGACCGGCGTCTCTGTGCGCGCGGGCCGGGACCGCGATCCTCGCGCTGGG431.7145135566188199No Hit
CAGCTGCGGCTAGTCCATTTGGAGCTCCTTCAAGCCGAAGCATCCAACAC381.5151515151515151No Hit
CGCTACCCTCCTCACACACCTCGGTCCCCTAACTACCCATTTCCCGGTCA331.3157894736842104No Hit
GCGGCGAAGACGCCTGAGCGTTCGCGCCCCTCGGGCGAGGACCCCACGCA250.9968102073365231No Hit
CTGAGCTTCCCAGCGAGTCCAGGGGCTGCTACAAAGCGCGGCGACGTCTC90.3588516746411483No Hit
CTAGCGCCTTCTGCCTCCTGGCCATTCCCTCGGTGGAGAACAGTGACGAT80.3189792663476874No Hit
CGACGCCCTTCTCTCCTCTTCGCCCCGCCCCTTCCTCGCCCCGCCTCCGC70.27910685805422647No Hit
CTGAGCTTCCCAGCGAGTCCTCACATTGCGGCGTGGTGGCCGACAAAACC70.27910685805422647No Hit
GTGCCTGCGTGGCGATCAGGCAACAGCTACCCTGGAGAACAGTGACGATC70.27910685805422647No Hit
CTAGCGCCTTCTGCCTCCTACTCCAGGGATGGGCTGTGGAGAACAGTGAC50.1993620414673046No Hit
AAGCGGAGGGCAGCAAAGCCCCCGAATGACTTCGCTTACCCCCACCCGCC30.11961722488038277No Hit
GTGCTGGCGGCAAGACGAGTTCTCGGAGGATGCTGCGGCGCGGGGCGCGT30.11961722488038277No Hit
CCCACCTACCTGCTCCTGTGACCAGGAATGGAGAACAGTGACGATCGCTT30.11961722488038277No Hit
GTGCTGGCGGCAAGACGAGGAGCCGGGATGTTGGAGAACAGTGACGATCG30.11961722488038277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)