FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000935655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000935655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508
Sequences flagged as poor quality0
Sequence length294-300
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCCTCTCGGCTCCTCTGGCTCTTGCGGGCCGGGCCGGGGCGGTC1756.977671451355662No Hit
CCTCTCGGGCCTGGGCTCTTCCCTCGCGGCCCGGGCCGGCCGGCGGCCCG1275.0637958532695375No Hit
GCGTCCAGGCGGAAGGAGCCTGGTGCCTCCTGCAGCCGCACCTGAGGAGA1214.824561403508771No Hit
CAGCCCATCCCTGGAGTAGGAGCTCCGGATGTGCCTCGGTGGTGTCCCCG1044.146730462519936No Hit
GGAAGACCAGGACCAGGAGATCACCGAGGGCGACCGCCAGGCCCCGGGCC933.7081339712918657No Hit
CGCGCGTCACAACCTCACCTGCAACGCGACCAGCCGCCAGCCTTCGGTCT923.6682615629984054No Hit
ACATCCCGGCTCCTCCTCTTCCTCCCTGGTGGCAGTGGCAGCTCCGGTGG883.508771929824561No Hit
CCTTCTCCAGCGTGGACCTGAGAGGCAGCCTGGCCGGAGCGCACGCCAGC813.2296650717703352No Hit
GGGATGCAGGGACAAGGAGATCACCGAGGGCGACCGCCAGGCCCCGGGCC803.1897926634768736No Hit
TCTCCGGCACCGTGAATGGGGAGCCCGAGTCGCTGGCGGCGGTCAGCCTG793.149920255183413No Hit
TCGTCTGTGTCTCCCTGTGGCTGTGCATCAGACCTCACGCGTTTAAAACG763.0303030303030303No Hit
GGGTAGCTGTTGCCTGAACGCCGGAGCGCTCCCCTCTCGCCGCTTGCCTC742.9505582137161084No Hit
GCTGCGGATGGGCACAGGCCATAGTGAGGGGGGCATGAGAGCCCCAGACC722.8708133971291865No Hit
CCCTTCCTGCCCTCTTTGATGCGGCCCCACTTCCTCTGGCAGGAACCCCC722.8708133971291865No Hit
GGGATGCAGGGACAAGGACAGAAAAGAGCCCGGCTCTTCCTCCCAGGGTC682.711323763955343No Hit
CCGAGGGAATGGCCAGGAGCCCCCCTCCCGGTAGAGTTTGCACTTTGTCT652.59170653907496No Hit
AGTTTAGGGATGATTCTCCAGCTCTGCACCCACCTGATCCTCTGATGGAG582.3125996810207337No Hit
GGAGATGTCCGTGGAGGCAAAGCCCGGGCCCCCGGGGCCGCCGGGAGACG572.272727272727273No Hit
GCACCCGGAGGACAAGCTCTTCAACACGTCTGTGGAGGTGCTGCCCTTCG542.15311004784689No Hit
GGGACCTTGGGAGCTGTGGTACAGAGGAGGCGCGGCCGCGAGTCCCGGCG461.8341307814992027No Hit
GGCTGAGGCAGACAAAGGAAAGGTAATGAGGTTAGGGCCCCCAGGCGGGC411.634768740031898No Hit
GAGGCGGAGCAGCAGAGCCCCCAGCCGGGGCAGCCGGGGCGACCGGGGCA401.5948963317384368No Hit
GCAGGAGGCCTTGCATTCCGGGGTCTCTGAGATGCTGGTGGGGGGCGCCC401.5948963317384368No Hit
GGCTCGGACGCCCAAGTTGGAGGGGACGGGGGTGGGGTCAATCCCTCCGC371.4752791068580542No Hit
GGCTCGGACGCCCAAGTTGGAGGGGACGGGGGTGGGGTCAATCTCTCCGC371.4752791068580542No Hit
TGCTTGCTAGTCCACCTTACCGCCGGATGGGCAGCCGCTGCTGAGCGCCT361.4354066985645932No Hit
TTAGCCCAAGCAGCACAGGATAGGGGGTCCAGCAGACCCTGCTTTTTGGC341.3556618819776716No Hit
CTGGCCATCCCACACAGGGATCAGGGTGGGAGGGCTGGAGGCTGCCTGTC331.3157894736842104No Hit
GCATTGTTCGCCGGATTTGTCCGCGGCGCGAATGGCCGCCGGGGGCCGGG250.9968102073365231No Hit
GCTGCGGATGGGCACAGGATAGGGGGTCCAGCAGACCCTGCTTTTTGGCT140.5582137161084529No Hit
CCGAGGGAATGGCCAGGAGGCAGAAGGCGCTAGTGGAGATGCTGCCGAGT120.4784688995215311No Hit
GGGTAGCTGTTGCCTGATCGCCACGCAGGCACTGGAGATGCTGCCGAGTC70.27910685805422647No Hit
CCTCTCGGGCCTGGGCTCTTTGATGCGGCCCCACTTCCTCTGGCAGGAAC60.23923444976076555No Hit
CCTTCCCTCCAAAGCCTGAAAGAGCTGAATTCTATTAAATCCTGGTTGGC50.1993620414673046No Hit
CAGCCCATCCCTGGAGTAGGAGGCAGAAGGCGCTAGTGGAGATGCTGCCG40.1594896331738437No Hit
GCGTCAGGCGGAAGGAGCCTGGTGCCTCCTGCAGCCGCACCTGAGGAGAG30.11961722488038277No Hit
TTCCTGGTCACAGGAGCAGGTAGGTGGGTGGAGATGCTGCCGAGTCTTTC30.11961722488038277No Hit
ACATCCCGGCTCCTCGTCTTGCCGCCAGCACTGGAGATGCTGCCGAGTCT30.11961722488038277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)