FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000936509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235944
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT31530.25510864569915787TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA27460.22217835112270456No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT25510.2064009372592933Illumina Single End PCR Primer 1 (96% over 25bp)
AATTATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT20540.16618875936126556Illumina Single End PCR Primer 1 (95% over 22bp)
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA12890.10429275112788282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)