FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000936511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT81210.40604999999999997TruSeq Adapter, Index 7 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA60110.30055No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44510.22255No Hit
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA26650.13324999999999998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA26250.13125TruSeq Adapter, Index 7 (97% over 39bp)
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG24940.1247No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA24500.1225No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT21670.10835No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC21120.10560000000000001No Hit
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA20370.10185000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)