FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000936569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA45410.22705No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC44710.22355No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC37900.1895No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG36210.18105000000000002No Hit
GGGGTCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGG29400.147No Hit
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA27680.1384No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC24350.12175000000000001No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT24300.1215TruSeq Adapter, Index 1 (97% over 36bp)
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA24080.1204No Hit
GTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGAAGCTCCATGA21690.10844999999999999No Hit
GTAGGCTTCTTTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAA20950.10475000000000001No Hit
CCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGAT20220.1011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)