FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000936573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC40310.20155No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA38100.1905No Hit
GTCCTGGTTGGTGTAACTCGCACCTCAACTCCAGAGTAGCCATCTTCAGC32930.16465No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG30940.1547No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT26580.13290000000000002TruSeq Adapter, Index 1 (97% over 36bp)
GTCGGGGTTGTAGCCAATTTTCTTAATGTAAGTGCTGACTTCCTTAACAA23390.11695No Hit
CTCTGTTCTTCAAGTTTCCCTTTGATTGATTTCATGTAATCTTTGATGTA23320.1166No Hit
CCCCTGTCATAAAAGGTTTTACTCTTTCTGGTCTCTGTTCTTCAAGTTTC22270.11134999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)