FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000936650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT516702.5835TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA157570.78785TruSeq Adapter, Index 5 (97% over 36bp)
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT69340.3467No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA50710.25355No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA42670.21334999999999998No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC39860.1993No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT39180.1959No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC36930.18465No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA32980.16490000000000002No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC27330.13665000000000002No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT27040.1352No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT25840.1292No Hit
GGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAAT24390.12195No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA24150.12075No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA21980.1099No Hit
GTCGCCTGTTCACCAAGGATTCTGTGCAGCATTTGGTGACTCTGTCACTT21110.10555No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT20930.10464999999999999No Hit
GCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGA20480.10239999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)