FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000936656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523187
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT95251.8205727588797123TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA19160.366217050500108TruSeq Adapter, Index 5 (97% over 36bp)
ATATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA13540.2587984793200137TruSeq Adapter, Index 5 (97% over 36bp)
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA11320.21636623234139993No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC9470.18100602652588846No Hit
CCTGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT9090.17374284911513474No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA8660.16552399046612398No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT8660.16552399046612398TruSeq Adapter, Index 5 (97% over 36bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC7350.1404851420237888No Hit
GCCCACTTTTCCTAGGTTTCTTGAGACCTCTACAAGAGTTGGAGTTGACA6800.12997264840296108No Hit
CCGGGGCATAAAAGTAAGGATGTCTTCTGGCAATTTCATATAAGTATTTT6540.12500310596402434No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC6450.12328287973516162No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT6210.11869560979152768No Hit
GCTCAGGCGAGCTACTGCCCATGCTTTGAAAGCTCTTTCTCCAAATTTTT6150.11754879230561921No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT6070.11601970232440792No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA5480.10474266371297451No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATATCGTAT5450.10416925497002028TruSeq Adapter, Index 5 (97% over 36bp)
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA5400.10321357373176321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)