FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000936856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC56730.28365No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA53430.26715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTAT30110.15055TruSeq Adapter, Index 1 (97% over 36bp)
GGGGTCTACAACCAGCATATCTTCATGGCTGTGAAATTCATGGCTGTGGA28060.1403No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG20730.10365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)