FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000936995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000936995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT227151.13575TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA51160.25579999999999997TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT45550.22775Illumina Single End PCR Primer 1 (96% over 25bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT38700.1935TruSeq Adapter, Index 5 (97% over 37bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT30720.15360000000000001TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT24020.1201Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)