FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000937003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000937003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT193920.9696TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT51220.2561Illumina Single End PCR Primer 1 (96% over 25bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT36180.1809TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA32910.16455TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT21570.10785Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)