FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000937091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000937091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG48010.24004999999999999No Hit
GTAGGCTTAATATAAAATAACCACATTAGTGAACATTATATCTCTTAGAA45680.2284No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC37320.18660000000000002No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA35780.1789No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA29810.14905000000000002No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTAT24430.12215TruSeq Adapter, Index 2 (97% over 38bp)
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT20540.10269999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)