FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000937428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000937428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA117930.58965No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC116710.58355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT76700.3835TruSeq Adapter, Index 9 (97% over 38bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC57180.2859No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACCACTGTATCTCGTAT33920.1696Illumina Single End PCR Primer 1 (96% over 25bp)
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC32830.16415No Hit
GCCCGGTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGC29640.1482No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT28780.1439No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG25920.1296No Hit
ACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGG21850.10925No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT21210.10605No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG21210.10605No Hit
CCCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCT20180.10089999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)