FastQCFastQC Report
Fri 9 Sep 2022
EGAF00000937918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000937918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT79780.39890000000000003TruSeq Adapter, Index 5 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA70530.35265No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT59240.29619999999999996Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC48550.24275No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT27630.13815No Hit
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT22840.1142Illumina Single End PCR Primer 1 (95% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA21970.10985TruSeq Adapter, Index 5 (97% over 37bp)
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT21610.10805000000000001No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA21390.10694999999999999No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC20160.1008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)