FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000937920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000937920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT95020.4751TruSeq Adapter, Index 5 (97% over 37bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA77440.3872No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC56190.28095No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT30780.1539Illumina Single End PCR Primer 1 (96% over 25bp)
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT28470.14235No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT25180.12589999999999998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA22850.11425TruSeq Adapter, Index 5 (97% over 37bp)
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA22470.11234999999999999No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC22300.11150000000000002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)