FastQCFastQC Report
Tue 8 Aug 2023
EGAF00000976693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000976693
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences52622223
Sequences flagged as poor quality0
Sequence length90
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT2659580.5054100432055103No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT2658570.5052181090867256No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG1762910.3350124528186504No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1610380.3060266002065325No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA1564030.2972185344583409No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1472700.27986274924189347No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA1423160.2704484757323916No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC1260100.2394615674066069No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG1168520.22205827374491574No Hit
CGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG1150710.2186737721057508No Hit
GCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCT1120760.21298226036554935No Hit
GCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGG1117290.21232284314556607No Hit
CGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCG1096010.20827892428641792No Hit
CTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGG1068510.20305299530960522No Hit
CGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTA995300.18914062220442493No Hit
GTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAAC968970.18413703275135296No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA954040.1812998284013961No Hit
GCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTC952690.18104328279707985No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC881640.16754138265880558No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC866900.16474028472723395No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG838080.15926351115953424No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC835500.1587732240046187No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA756330.14372824956482738No Hit
GTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTC751680.14284459248329362No Hit
CCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGC741900.14098606210535802No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG727200.13819256552502543No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGA721990.13720248952614564No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC721970.13719868885052613No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC720750.13696684763773662No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT719750.13677681385676163No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT717760.13639864663262136No Hit
CCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGA708650.13466743888793906No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG708450.13462943213174403No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG695240.13211908588506419No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG683760.12993749807947108No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT682180.1296372447055306No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA678410.12892081735125482No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG673470.12798205047323827No Hit
GCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCA662230.1258460707750792No Hit
CCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCT659150.12526076672967618No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG657940.1250308258546964No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA655810.12462605390121963No Hit
CGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTC644020.1223855556235243No Hit
TCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGG628380.11941342728907518No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC623380.11846325838420016No Hit
CAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAA615920.1170456063781266No Hit
GTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTCTCGTGCC605030.11497613850330876No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGC605030.11497613850330876No Hit
CGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGC603960.11477280235766552No Hit
GTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCT592550.11260451691674067No Hit
CCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAG574940.10925802203377079No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGT557480.10594003221794715No Hit
CGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGA553490.10518179743185688No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT540970.10280257449404978No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTA538210.10227808125855876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)