FastQCFastQC Report
Tue 28 Mar 2023
EGAF00000976697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000976697
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences53422223
Sequences flagged as poor quality0
Sequence length90
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT2112780.3954870990673675No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT2054000.3844841874139157No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA1574320.29469383930354226No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG1397440.26158402281387655No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1312690.24571983835266456No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA1251090.23418905649059193No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG1107320.20727703525179028No Hit
GCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCT1102680.20640848285178995No Hit
GCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGG1036770.19407092063540674No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC1013190.1896570271888536No Hit
CGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG935900.17518926533626278No Hit
CGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCG929120.1739201305793658No Hit
GCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTC927900.17369176119833127No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC881320.16497254335522504No Hit
GTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAAC855110.16006634542332693No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC852090.15950103761125778No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC713210.13350436577676672No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG712640.13339766860693908No Hit
CGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTA708980.13271256046383542No Hit
GTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTC696420.13036147896728295No Hit
GCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCA661820.12388477357072918No Hit
TCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGG617650.11561667884917481No Hit
CCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGC606780.11358194510176037No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG605110.11326934111296717No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC595490.11146859238710452No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG593930.11117657908020788No Hit
CTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGG592710.11094820969917332No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT586700.10982320971555228No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA551860.10330157919486053No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA546690.10233381714572229No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG543100.10166181216382554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)