FastQCFastQC Report
Wed 15 May 2024
EGAF00000976698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000976698
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences51377778
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT2055350.40004649480948745No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT1753550.34130514558259023No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1738910.3384556646260568No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA1344830.26175324281248596No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG1297800.2525994798762998No Hit
CTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGG1276720.24849653871757554No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1151970.22421561321706052No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC1073540.20895025861180686No Hit
GCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGA1045350.2034634506770612No Hit
CCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGA1019700.19847101990280702No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA1019100.19835423789639173No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA982110.1911546272008883No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA980130.19076924657971778No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC969350.1886710631977895No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC931340.1812729230913801No Hit
GCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCT929910.18099459264275694No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG928770.18077270683056787No Hit
GCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTC913240.17774999923118512No Hit
CGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG886970.17263689371696844No Hit
GCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTC862930.167957827993262No Hit
CAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAA844730.16441544046533113No Hit
GCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGG831050.16175281071906222No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG818380.15928676401692576No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTA801840.15606747337341056No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGC799450.15560229171452294No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT790620.1538836498534444No Hit
CGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTA748630.1457108557711468No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT720340.1402045841686653No Hit
TTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCA682990.13293490426931268No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG646480.1258287191789415No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC623980.12144939393836768No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC622270.12111656522008406No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC621810.12102703234849899No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC618850.12045090778351683No Hit
CGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGC615900.11987672958530826No Hit
GTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTC612960.11930449775387328No Hit
TGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACC611840.11908650467523138No Hit
GTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAAC609970.11872253408857036No Hit
CGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTC608580.11845198910704158No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT605110.11777659983660639No Hit
CCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACT589370.1147130185349783No Hit
GCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAAC562110.10940722271017637No Hit
CCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAG554180.1078637538587208No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA549440.1069411760080399No Hit
TCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT549430.1069392296412663No Hit
CCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAG546640.10639619331143514No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT532670.10367711892873219No Hit
CCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCT531650.10347858951782617No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG530130.10318274176824074No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG529860.10313018986535384No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG517140.10065441132934942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)