FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001010578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001010578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25915104
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG660520.2548783906095843No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT633640.2445060610214028No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA395800.15272946618311853No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT360780.13921611119137317No Hit
GGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCA360660.1391698061485688No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG355070.13701276290459802No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACA348560.13450071433246033No Hit
GCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAG347630.13414185025072636No Hit
CCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGT342930.13232823607422145No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA339720.13108957617920422No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG339080.1308426159509142No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA327750.12647064815946718No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG323970.12501203931112914No Hit
AGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGC319560.12331032898806812No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGG310230.11971011191002745No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA307650.11871455348973325No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA300940.1161253298462549No Hit
GTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTAATGTGGTGGGTGAGTG293580.1132852872209195No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC275600.10634724830739634No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT267810.10334127927867857No Hit
CTTTAGTGTTGTGTATGGCTATCATTTGTTTTGAGGTTAGTTTGATTAGT262760.1013926087273275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)