FastQCFastQC Report
Wed 6 Sep 2023
EGAF00001010726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001010726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63497903
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1855840.29226791946184427No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1563190.24617978329142617No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTAT1026330.16163210933123257TruSeq Adapter, Index 8 (97% over 37bp)
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT874840.13777462855741868No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT810030.1275679922847216No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC762390.12006538231664123No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG750410.11817870583852196No Hit
ACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTC679350.1069877850926825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)