FastQCFastQC Report
Mon 19 Dec 2022
EGAF00001073665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001073665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95158846
Sequences flagged as poor quality0
Sequence length250
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5227900.5493866539743452Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3790210.3983034851011119Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1806900.1898825044599637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)