FastQCFastQC Report
Fri 30 Dec 2022
EGAF00001073669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001073669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93297006
Sequences flagged as poor quality0
Sequence length250
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1742070.18672303374880003No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1103880.11831890939779997Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT1003280.10753614108474177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)